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<blockquote data-quote="Alastair" data-source="post: 13170" data-attributes="member: 13"><p>Good advice, you and I do the same thing. Cotton swab with saline, air gap, do not take mucus, all that. For the antibiotic I use Gentamycin which does a good job. Re the saline killing sperm, study I read does not support that.</p><p></p><p>If you watch Sue Coby's videos you will see she gets quite a bit of saline in the sperm, a lot more than I do, she does not indicate it as a problem other than to say to factor that in when measuring quantity to put into the queen.</p><p></p><p>The guy who trained me uses a special mix with several antibiotics plus a food for the sperm. However I just use saline with gentamycin. Since it has always done a good job for me in the past I do not think it would suddenly be the problem now.</p><p></p><p>What I am going to change is deliberately adding some saline to the semen if it is an unduly hot day when I am collecting. I have used semen diluted with saline in the past and those queens have been able to head up full production colonies just as good as a naturally mated queen so I don't think it is a problem. Of course I do not know for sure, but I think the issue was dried thick sperm that the queens had problems properly transferring to the spermatheca. It is the only difference between that days inseminations, and the rest of the days with normal results.</p></blockquote><p></p>
[QUOTE="Alastair, post: 13170, member: 13"] Good advice, you and I do the same thing. Cotton swab with saline, air gap, do not take mucus, all that. For the antibiotic I use Gentamycin which does a good job. Re the saline killing sperm, study I read does not support that. If you watch Sue Coby's videos you will see she gets quite a bit of saline in the sperm, a lot more than I do, she does not indicate it as a problem other than to say to factor that in when measuring quantity to put into the queen. The guy who trained me uses a special mix with several antibiotics plus a food for the sperm. However I just use saline with gentamycin. Since it has always done a good job for me in the past I do not think it would suddenly be the problem now. What I am going to change is deliberately adding some saline to the semen if it is an unduly hot day when I am collecting. I have used semen diluted with saline in the past and those queens have been able to head up full production colonies just as good as a naturally mated queen so I don't think it is a problem. Of course I do not know for sure, but I think the issue was dried thick sperm that the queens had problems properly transferring to the spermatheca. It is the only difference between that days inseminations, and the rest of the days with normal results. [/QUOTE]
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