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Breeding Bees in New Zealand
Artificial Insemination
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<blockquote data-quote="Breeder of queens Koss" data-source="post: 13169" data-attributes="member: 449"><p>It's hard to advise something when you haven't seen the process. I can guess what the reason might be....</p><p>1. You do not know the age of the drone from which you are collecting sperm. Perhaps you come across a drone that is too old; its sperm is darker and more viscous. In a normal drone it is lighter.</p><p>You need to be careful not to collect mucus together with semen.</p><p>2. When I worked with saline, I added an antibiotic to it. There must be an air cushion in the capillary to separate the solution in the system from the semen. Next, when collecting sperm into the capillary, use sterile cotton swabs soaked in saline solution with an antibiotic. They need to wipe the tip of the capillary from dirt, preferably after each drone. If you need to take a short break from work, do it like in the photographs - semen, air gap, saline solution. For saline solution, I use a regular syringe with a needle. They are convenient for moistening cotton swabs.</p><p>Direct contact of saline solution and sperm is not recommended, because it kills sperm, but I personally did not check it, I followed the recommendations.</p><p>3. Reduce the insemination dose.</p><p>After insemination, queens that are inseminated with large doses of sperm require maintenance by bees.[ATTACH=full]1724[/ATTACH]</p></blockquote><p></p>
[QUOTE="Breeder of queens Koss, post: 13169, member: 449"] It's hard to advise something when you haven't seen the process. I can guess what the reason might be.... 1. You do not know the age of the drone from which you are collecting sperm. Perhaps you come across a drone that is too old; its sperm is darker and more viscous. In a normal drone it is lighter. You need to be careful not to collect mucus together with semen. 2. When I worked with saline, I added an antibiotic to it. There must be an air cushion in the capillary to separate the solution in the system from the semen. Next, when collecting sperm into the capillary, use sterile cotton swabs soaked in saline solution with an antibiotic. They need to wipe the tip of the capillary from dirt, preferably after each drone. If you need to take a short break from work, do it like in the photographs - semen, air gap, saline solution. For saline solution, I use a regular syringe with a needle. They are convenient for moistening cotton swabs. Direct contact of saline solution and sperm is not recommended, because it kills sperm, but I personally did not check it, I followed the recommendations. 3. Reduce the insemination dose. After insemination, queens that are inseminated with large doses of sperm require maintenance by bees.[ATTACH type="full"]1724[/ATTACH] [/QUOTE]
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