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<blockquote data-quote="Alastair" data-source="post: 13166" data-attributes="member: 13"><p>I had a look last friday and only 6 of them were laying eggs, and most of the others were quite small not even close to laying yet, which is rather worrisome, normally almost all of them would be laying within a week. So I am having another look at them tomorrow, which will be two weeks, anything not laying by then will be a fail.</p><p>As I mentioned already the semen was very thick and I suspected it had dried some, the heat was fierce when I was collecting it, this may be the reason. I have done some more reading of Sue Coby and she thinks the semen can dry so fast even in the capillary that she recommends taking a little saline up the pipette between every drone. I have never had this problem before but I have never collected semen in such hot conditions. From now I will be taking a little saline between each drone, and up the amount to each queen accordingly. I have experimented with adding saline before, and even diluting it 50% does not cause any problems.</p><p></p><p>Anyhow I will update the thread tomorrow after I check them but I think it may be a poor result.</p></blockquote><p></p>
[QUOTE="Alastair, post: 13166, member: 13"] I had a look last friday and only 6 of them were laying eggs, and most of the others were quite small not even close to laying yet, which is rather worrisome, normally almost all of them would be laying within a week. So I am having another look at them tomorrow, which will be two weeks, anything not laying by then will be a fail. As I mentioned already the semen was very thick and I suspected it had dried some, the heat was fierce when I was collecting it, this may be the reason. I have done some more reading of Sue Coby and she thinks the semen can dry so fast even in the capillary that she recommends taking a little saline up the pipette between every drone. I have never had this problem before but I have never collected semen in such hot conditions. From now I will be taking a little saline between each drone, and up the amount to each queen accordingly. I have experimented with adding saline before, and even diluting it 50% does not cause any problems. Anyhow I will update the thread tomorrow after I check them but I think it may be a poor result. [/QUOTE]
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