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JohnF last won the day on August 4 2015

JohnF had the most liked content!

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About JohnF


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    dnature diagnostics & research Ltd
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    Bee Research
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  1. $85 is for the nosemas and Lotmaria - more if wanting the full panel as @Rob Atkinson showed. Wouldnt suggest doing 2 bees per hive - you’re going to dilute out or ‘homogenise’ your results. Unless of course you only have about 20 bees left ! I haven’t looked it up lately but there was a thought that BQCV is a mycovirus - ie a virus that also infects fungi - in this case, nosema apis.
  2. There are plenty of people doing science here, Alastair. Plus the scientists like @Otto and @Dave Black, along with the Plant and Food guys. The gradings are somewhat arbritrary and cover the Cq range that we see - so not based on NZ averages but more, based on a high, low, moderate Cq across the range. Yes, the comb sterilisation method was confirmed by James Sainsbury at Plant and Food, using an overseas description as I understand it. Freezing combs does not work (well, except for wax moth!) I have a few answers and opinions to your questions Phil buts it late and it'll have to wat. But what can you do (your question at the end). Vote yes to a research le . . .oh, too late. Well, let's hope there might be a second chance for research levy or funding somewhere. Because the free train has come to the end of the line . . . .
  3. Thanks Alastair - credit to Rebecca (not me!) and her handling of your samples Yes, nosemas are fungal-like . . microsporidians, if you want to be more precise. Lotmaria passim is a trypanosome Cororapa is the name we gave to the syndrome. Rather than hammering Coromandel (where it was first described), we dubbed it COROmandel/WairaRAPA. . . yes, see what we did there? Since found in Taranaki, East Coast (including a couple of my own hives a couple of seasons ago) and other areas. Plant and Food studied Nosema ceranae but I believe it to be the combination of both nosema species . . .which has been described as being doubly bad (or more correctly, life span clearly shortened with both vs 1 . . .vs neither) This study was done by @TammyW in our lab a couple of years ago Craig - we miss her but she's doing cool things at Scion in Rotorua after completing her Masters. Thanks to the beekeepers on this forum who supported that work ! One thing is that the levels cannot be absolute. Hives with high nosema levels in spring can go on and survive if caught in time. But when we have tested bees individually from a dwindled hive then we've seen very high levels as well. Testing of bees will also show whether considering comb sterilisation is worthwhile (50 deg for 2 hours)
  4. Only thing with that Phil is that you can not (reliably) tell Nosema ceranae from Nosema apis. And you will need quite a level to see it at all ie a 'no nosema seen' slide does not mean no nosema !
  5. No, not due to the typical signature of cororapa We do see quite a bit of nosema apis - a drain on the hives (Mark Goodwin estimated it accounted for 15% of a potential crop). But the levels in these samples were very high - much higher than we normally see. I wonder if staples were dry enough and the glycerine is attracting water, does the dampness encourage the nosema apis?
  6. *coughing again* The original results were for our Nosema duo (both nosema species) and we include Lotmaria in that as well. The results went to James just over a couple of weeks ago and 2 days after we received them. Just in case anyone was wondering The samples tested had very high levels of Nosema apis - but no Nosema ceranae. We kept the extracted material and tested them this week out of interest, to see if any viral culprits at work. We only test 3 of the samples but one of them did have very high levels of Deformed Wing Virus - possibly a colony moving away from the staples?? I have no idea there The 3 targets are $85 ex GST per sample. Testing for 5 viruses and nosemas/trypanosomes is about twice that (at a single sample rate)
  7. *sigh* must be getting past bedtime! I just wanted to make sure the conclusion WASN'T that we/dnature hadn't returned the results. Is this correct @yesbut ? Or is my double negative poor grammar and I should not be so circumspect and say 'the conclusion should be we returned results'.
  8. No, because we're all good friends here, I just wanted to make sure the conclusion was that we/dnature hadn't returned the results ! I can put them here but that requires @jamesc's permission. One thing this does highlight though is my comment a short while ago about beekeepers wanting research for free. There are a number of people who may well be seeing something similar as James or Alastair or others and are wanting to see their results . . .and will apply those results as 'so that's what's happening in my hives'. I heard on the radio when the Cororapa first broke out and the discovery we made of Lotmaria passim in the country. One beekeeper was blaming this new pathogen for his hive losses. Had he got them tested? No - because we had the only test and he sure hadn't talked with us.
  9. All good Alastair, have replied to you. Rapidly dwindling hives we'd test for nosemas (both N. ceranae and N. apis) as well as Lotmaria.
  10. If memory serves, wasn't there a weight even for the 'squeezed' staples? @Alastair and @jamesc do you have the document that reviewed the first 100-odd pages of the thread? Is there a permalink for it somewhere?? (have not used the staples before)
  11. Oooh, science ! Sorry I'm late Are there dead bees @Alastair and others? It would be useful if those affected wrote a case history of a typical hive eg. 3 weeks ago, 2 boxes - now down to handful of bees with food, pollen on board. Queen-right? Being robbed? Not being robbed? etc I'd be more worried that they'd be a waste of money. We don't like testing unless there is a good reason to do it or something suspicious *cough* Whispers: "Bayvarol @M4tt, not Apivar. We test for resistance mutations for Bayvarol/Apistan" Can also be due to pathogens - a recent paper was mentioned here where shotgun brood queens shifted onto new frames started laying up a storm. So 'queen problems' might actually be pathogen/comb problems. Oksana Borowik wrote a good review of this in a recent NZ Beekeeper issue
  12. Nothing new - it was presented several years ago that there were Australian species with higher levels of DHA, MGO and leptosperin. What is the ‘best manuka’? An active honey with the highest MGO? Ah oh no wait, the one with the highest MGO *and* leptosperin . . . Ah oh again.
  13. Worse Philbee, its becoming other countries telling us "you have a problem, fix it" (nosemas in bees, AFB in honey, manuka in honey) . . .and to which the country responds "well, what have you done about it to date? Oh, $300 million in exports but zero in industry funding? Back to splintered groups?" With the answer of "yeah . . .nah"
  14. If you hit the 'quote' button at the end of a post, it will do the above (ie quote the post so you don't have to copy it out). Now, where was I ? Oh yes . . . you're missing my point. The call for help is not from researchers at the moment, it is from beekeepers who cant sell honey that used to have a market in being blended into "manuka". Nothing to do with hype and gloss, just stark reality now. And perhaps more stark for some than others who have seen this before
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